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stat3  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology stat3
    Stat3, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 4603 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stat3/Stat3+Antibody/pm42864331-489-104-107
    Average 96 stars, based on 4603 article reviews
    stat3 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: O-GlcNAcylation regulates microglial neuroinflammation in Parkinson's disease.
    Article Snippet: Western Blotting AR TI CL E IN P RE SS Equal amounts of protein (30 μg) were separated on 10% or 15% SDS-PAGE gels and transferred to nitrocellulose membranes (Amersham). .. Membranes were blocked with 5% nonfat milk in PBST for 1 h at room temperature, then incubated overnight at 4°C with primary antibodies (1:1,000) against various targets, including O-GlcNAc (sc-59623, RL2, Santa Cruz, Dallas, TX, USA), OGT (sc-74546, Santa Cruz), OGA (14771-1-AP, Proteintech, Rosemont, IL, USA), GFAT2 (A15374, Abclonal, Woburn, MA, USA) tyrosine hydroxylase (T2928,TH, Sigma-Aldrich), β-actin (sc28365, Santa Cruz), p-p65 (ab86229, Abcam, Cambridge, UK), p65 (sc-8008, Santa Cruz), p-IκB (sc-8404, Santa Cruz), IκB (sc-371, Santa Cruz), iNOS (610328, BD Bioscience, San Jose, CA, USA), COX2 (A3560, Abclonal), p-CREB (ab32096, Abcam), CREB (sc-377154, Santa Cruz), TLR4 (A0007, Abclonal), Arg1 (A4923, Abclonal), CD163 (A26411PM, Abclonal), CD86 (A16805, Abclonal), CD63 (A6554, Abclonal), c-Rel (sc-6955, Santa Cruz), p-STAT3 (9145, Cell Signaling Technology, Danvers, MA, USA), STAT3 (sc-8019, Santa Cruz), p-ERK (9101, Cell Signaling Technology), ERK (A16686, Abclonal), PPARγ (sc-7273, Santa Cruz), NLRP3 (sc134306, Santa Cruz), ASC (sc-514414, Cell Signaling Technology), cleaved caspase 1 (89332, Cell Signaling Technology), and caspase 1 (24232, Cell Signaling Technology). .. Membranes were washed and incubated with horseradish peroxidase-conjugated secondary antibodies (Invitrogen) for 1 h at RT.

    Article Title: Isolated naive pluripotent stem cells and methods of generating same
    Article Snippet: .. Blots were incubated with the following antibodies in 3% BSA/TBST or PBST: pSTAT3 (9318; 1:1,000; Cell Signaling), STAT3 (C-20; 1:1,000; Santa Cruz), po-Catenin (9561; 1:750; Cell Signaling), β-Catenin (610153; 1:2,000; BD Biosciences), HSP90a (CA1016; 1:5,000; Calbiochem), pJNK (9251; 1:500; Cell Signaling), JNK (SC-571; 1:100; Santa Cruz), pp38 (9215; 1:100; Cell Signaling), p38 (9212; 1:1,000; Cell Signaling), pERK (E4; 1:100; Santa Cruz), ERK1,2 (C-14; 1:100; Santa Cruz), KLF4 (AF3158; 1:200; R&D), OCT4 (H-134; 1:1,000; Santa Cruz), Nanog (397A; 1:1,000; Bethyl). ..

    Western Blot:

    Article Title: Inhibition of CCL4 prevents renal inflammation and fibrosis in acute kidney injury
    Article Snippet: Membranes were blocked with 1% BSA217 solution for 1 hour then incubated with antibodies against CCL4 (Santa Cruz,218 sc-393441, 1:1000, Dallas, TX, USA), kidney injury molecule-1 (KIM-1; R&D219 Systems, AF1817, 1:1000, Minneapolis, USA), phosphorylated (p)-STAT3 (Cell220 Signaling Technology, #9145S, 1:1000, Danvers, MA, USA), STAT3 (Cell Signaling221 Technology, #9139S, #12640, 1:1000, Danvers, MA, USA) , transforming growth222 factor-β (TGF-β; Cell Signaling Technology, #3711S, 1:1000, Danvers, MA, USA),223 p-Smad2/3 (Cell Signaling Technology, #8828S, 1:1000, Danvers, MA, USA),224 Smad2/3 (Cell Signaling Technology, #3102S, 1:1000,Danvers, MA, USA), collagen225 1 (Cell Signaling Technology, #91144S, 1:1000, Danvers, MA, USA), IL-1β (Santa226 Cruz, sc-7884, 1:1000, Dallas, TX, USA), IL-6 (Cell Signaling Technology, #12153S,227 1:1000, Danvers, MA, USA), and TNF-α (Cell Signaling, 3707S, 1:1000, Danvers,228 MA, USA). .. The immunoblotting expression of KIM-1, p-STAT3, STAT3, TGF-β,229 p-Smad2/3, Smad2/3, collagen 1, IL-1β, IL-6, and TNF-α was normalized to the actin230 expression determined using an anti-β-actin antibody (Santa Cruz Biotechnology,231 sc-81178, Dallas, TX, USA; 1:10,000).232 2.13 Statistical analysis233 All raw data were analyzed by Shapiro-Wilk normality test. ..

    Expressing:

    Article Title: Inhibition of CCL4 prevents renal inflammation and fibrosis in acute kidney injury
    Article Snippet: Membranes were blocked with 1% BSA217 solution for 1 hour then incubated with antibodies against CCL4 (Santa Cruz,218 sc-393441, 1:1000, Dallas, TX, USA), kidney injury molecule-1 (KIM-1; R&D219 Systems, AF1817, 1:1000, Minneapolis, USA), phosphorylated (p)-STAT3 (Cell220 Signaling Technology, #9145S, 1:1000, Danvers, MA, USA), STAT3 (Cell Signaling221 Technology, #9139S, #12640, 1:1000, Danvers, MA, USA) , transforming growth222 factor-β (TGF-β; Cell Signaling Technology, #3711S, 1:1000, Danvers, MA, USA),223 p-Smad2/3 (Cell Signaling Technology, #8828S, 1:1000, Danvers, MA, USA),224 Smad2/3 (Cell Signaling Technology, #3102S, 1:1000,Danvers, MA, USA), collagen225 1 (Cell Signaling Technology, #91144S, 1:1000, Danvers, MA, USA), IL-1β (Santa226 Cruz, sc-7884, 1:1000, Dallas, TX, USA), IL-6 (Cell Signaling Technology, #12153S,227 1:1000, Danvers, MA, USA), and TNF-α (Cell Signaling, 3707S, 1:1000, Danvers,228 MA, USA). .. The immunoblotting expression of KIM-1, p-STAT3, STAT3, TGF-β,229 p-Smad2/3, Smad2/3, collagen 1, IL-1β, IL-6, and TNF-α was normalized to the actin230 expression determined using an anti-β-actin antibody (Santa Cruz Biotechnology,231 sc-81178, Dallas, TX, USA; 1:10,000).232 2.13 Statistical analysis233 All raw data were analyzed by Shapiro-Wilk normality test. ..

    Control:

    Article Title: STING–STAT3–SOX18 Axis Drives EndMT and Epigenetic Reprogramming in SAVI Lung Fibrosis
    Article Snippet: .. siRNA plasmids for control (sc-37007), STAT3 (sc-29493), and STAT1 (sc-44123) were purchased from Santa Cruz. .. HLMEC cells were treated continuously with 20 μg/ml of the STING ligand Cyclic [G(2’,5’)pA(3’,5’)p] (2’3’cGAMP) (Invivogen, Cat# tlrl-nacga23-5) and/or 1000 units/ml IFNβ (human IFN beta 1a, PBL Assay Science Cat# 11410-2) for 8 hours, 1, 3, and 5 days.

    Article Title: Exploring the immunomodulatory effects of environmental contaminants on autoimmune patients: An in vitro approach
    Article Snippet: Following washes, membranes were incubated with HRP-conjugated secondary antibodies, and signals were detected using PierceTM ECL substrate (Thermo Scientific, Waltham, MA, USA) on a ChemiDocTM MP imaging system (Bio-Rad, Hercules, CA, USA). .. Membranes were stripped and re-probed for total AKT, NFκB p65, p38 MAPK, STAT1, STAT3, and β-actin (Cell Signaling Technology, Danvers, MA, USA; Santa Cruz Biotechnology, Dallas, TX, USA) as a loading control. ..

    Cell-Signaling:

    Article Title: LGR5-positive fibroblasts orchestrate skin mechanoadaptation through JAK1 modulation.
    Article Snippet: .. The following primary antibodies were employed: GAPDH (Rabbit, 1:3000, Epizyme, LF206), LGR5 (Rabbit, 1:1000, abcam, ab75850), YAP (Rabbit, 1:1000, Cell Signaling Technology, 14074), COL1A1 (Rabbit, 1:1000, Cell Signaling Technology, 72026), COL3A1 (Rabbit, 1:1000, Cell Signaling Technology, 63034), ELN (Mouse, 1:1000, Santa Cruz Biotechnology, sc-58756), αSMA (Rabbit, 1:1000, Cell Signaling Technology, 19245), Lamin A/C (Rabbit, 1:3000, Proteintech, 10298-1), β-catenin (Rabbit, 1:1000, Cell Signaling Technology, 8480), JAK1 (Mouse, 1:1000, Cell Signaling Technology, 50996), pJAK1 (Rabbit, 1:1000, Cell Signaling Technology, 3331), STAT1 (Rabbit, 1:1000, Cell Signaling Technology, 9172), pSTAT1 (Rabbit, 1:1000, Cell Signaling Technology, 9167), STAT2 (Rabbit, 1:1000, HUABIO, ET1702-18), pSTAT2 (Rabbit, 1:1000, Cell Signaling Technology, 4441), STAT3 (Mouse, 1:1000, Santa Cruz Biotechnology, sc-8019), pSTAT3 (Rabbit, 1:1000, Cell Signaling Technology, 9145), MMP3 (Mouse, 1:1000, Proteintech, 66338-1), DYKDDDDKTag/Flag (Rabbit, 1:1000, Cell Signaling Technology, 14793), Myc Tag (Rabbit, 1:1000, Cell Signaling Technology, 2276), c-FOS (Rabbit, 1:1000, Abcam, ab222699), c-JUN (Rabbit, 1:1000, Cell Signaling Technology, 9165), TAZ (Mouse, 1:1000, Santa Cruz Biotechnology, sc-518026), β-actin (Mouse, 1:3000, Epizyme, LF201), p16 (Mouse, 1:1000, Santa Cruz Biotechnology, sc-1661). ..

    other:

    Article Title: Aberrant expression of the testis kinase TSSK6activates FAK–STAT3 signaling to promote tumorigenic growth
    Article Snippet: Antibodies used: TSSK6 (sc-514076, Santa Cruz) (1:500 IB), STAT3 (sc-8019, Santa Cruz (1:1000 IB), STAT3 (9139, CST)(1:1000 IB, 1:300 IF), pSTAT3 (Ser727) (9134, CST)(1:1000 IB), pSTAT3 (Y705) (9131, CST), Vinculin (sc-73614, Santa Cruz) (1:2000 IB), Beta-Tubulin (2128, CST) (1:5000), V5-tag (anti-rabbit,13202, CST) (1:5000 IB), V5-tag (anti-mouse, 46-0705, Invitrogen)(1:2000 IB), PLEKHA7 (PA5-85686, Invitrogen) (1:1000 IB), MEF2C (ab227085, Abcam)(1:5000 IB), pFAK (Y397) (44-624G, Invitrogen) (1:1000 IB, 1:200 IF), FAK (3286, CST)(1:1000 IB).



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